A handy system for gel electrophoresis.
نویسندگان
چکیده
منابع مشابه
Pulsed field gel electrophoresis using a double-decker gel system.
Pulsed field gel electrophoresis (PFGE) is capable of resolving large DNA molecules of up to two million nucleotides and is becoming a widely used technique in DNA studies (1—3). Three factors can potentially limit PFGE in a laboratory: 1. the cost of the apparatus, 2. reproducibility of electrophoretic conditions, and 3. unusually long electrophoretic time. To alleviate some of these problems ...
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Gel electrophoresis, a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. Manual or semiautomatic image processing presents a bottleneck for further development and leads to reproducibility issues. In this paper, we present a fully automated system with high accuracy for analyzing DNA and proteins. The proposed algorith...
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Electrophoresis is the migration of charged particles or molecules in an electric field. This occurs when the substances are in aqueous solution. The speed of migration is dependent on the applied electric field strength and the charges of the molecules. Thus, differently charged molecules will form individual zones while they migrate. In order to keep diffusion of the zones to a minimum, elect...
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متن کاملGel Electrophoresis
Electrophoresis is the main metod for separating large polyelectrolyte molecules, primarily used on DNA, in biochemistry and medicine research. An overwiev of the models of physical mechanisms that are behind this phenomena will be presented. The prominent role is taken by reptation mechanisms, by which large exible polyelectrolytes thread their way through the pores of the gel matrix. There a...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1986
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.35.3_t7